{"id":543,"date":"2022-09-01T07:36:00","date_gmt":"2022-09-01T05:36:00","guid":{"rendered":"http:\/\/upturn.site\/pl\/?p=543"},"modified":"2023-04-06T13:39:07","modified_gmt":"2023-04-06T11:39:07","slug":"publikacja-w-talanta","status":"publish","type":"post","link":"https:\/\/upturn.site\/pl\/publikacja-w-talanta\/","title":{"rendered":"Publikacja w Talanta"},"content":{"rendered":"\n<h4 class=\"wp-block-heading has-theme-palette-8-background-color has-background\">Boron-doped carbon nanowalls for fast and direct detection of cytochrome C and ricin by matrix-free laser desorption\/ionization mass spectrometry.<\/h4>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h5 class=\"wp-block-heading\"><strong>Abstrakt<\/strong><\/h5>\n\n\n\n<p>Detecting proteins via surface assisted laser desorption\/ionization mass spectrometry (SALDI-MS) method is still highly challenging, and only few examples of nanomaterials have been demonstrated to perform such detection so far. In this study, carbon nanowalls (CNWs), vertically aligned graphene sheet-based materials, presenting specific morphology, dimensions, and boron doping levels have shown improved performances for both qualitative and quantitative detection of Cytochrome C under optimized experimental conditions. Boron doped carbon nanowalls (B-CNWs) with a [B]\/[C] ratio of 5000 ppm and growing time of 4 h have shown the best performance in terms of signal intensity and reliability. Then, the detection of ricin, a ribosomal-inhibiting protein (RIP) classified as category B bioterrorism agent by CDC (Centre of Disease and Control and Prevention), was performed. For the first time, direct SALDI-MS detection of ricin B chain was reported without tedious sample preparation steps or database interrogation, and results were obtained within few minutes and a limit of detection (LOD) of 0.5 pmol\/\u03bcl was obtained. Thanks to the introduction of galactosamine residues on B\u2013CNW, we were able to selectively detect ricin B chain protein in complex media such as serum and soft drinks with enhanced signal intensity. B-CNWs are not toxic and are adaptable to any commercial MALDI-TOF mass spectrometer, showing their great potential as SALDI based materials.<\/p>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"427\" src=\"https:\/\/upturn.site\/pl\/wp-content\/uploads\/2023\/04\/06-1024x427.jpg\" alt=\"\" class=\"wp-image-544\" srcset=\"https:\/\/upturn.site\/pl\/wp-content\/uploads\/2023\/04\/06-1024x427.jpg 1024w, https:\/\/upturn.site\/pl\/wp-content\/uploads\/2023\/04\/06-300x125.jpg 300w, https:\/\/upturn.site\/pl\/wp-content\/uploads\/2023\/04\/06-768x320.jpg 768w, https:\/\/upturn.site\/pl\/wp-content\/uploads\/2023\/04\/06-1536x641.jpg 1536w, https:\/\/upturn.site\/pl\/wp-content\/uploads\/2023\/04\/06-2048x854.jpg 2048w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p><a href=\"https:\/\/doi.org\/10.1016\/j.talanta.2022.123778\">Pe\u0142na tre\u015b\u0107<\/a><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n","protected":false},"excerpt":{"rendered":"<p>Boron-doped carbon nanowalls for fast and direct detection of cytochrome C and ricin by matrix-free laser desorption\/ionization mass spectrometry.<\/p>\n","protected":false},"author":1,"featured_media":495,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[12],"tags":[],"_links":{"self":[{"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/posts\/543"}],"collection":[{"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/comments?post=543"}],"version-history":[{"count":1,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/posts\/543\/revisions"}],"predecessor-version":[{"id":545,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/posts\/543\/revisions\/545"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/media\/495"}],"wp:attachment":[{"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/media?parent=543"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/categories?post=543"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/tags?post=543"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}