{"id":616,"date":"2023-09-29T16:24:00","date_gmt":"2023-09-29T14:24:00","guid":{"rendered":"http:\/\/upturn.site\/pl\/?p=616"},"modified":"2023-11-29T13:58:49","modified_gmt":"2023-11-29T11:58:49","slug":"publikacja-w-microchimica-acta-2","status":"publish","type":"post","link":"https:\/\/upturn.site\/pl\/publikacja-w-microchimica-acta-2\/","title":{"rendered":"Publikacja w Microchimica Acta"},"content":{"rendered":"\n<h4 class=\"wp-block-heading has-text-align-left has-theme-palette-8-background-color has-background\">Boron-doped diamond nanosheet volume-enriched screen-printed carbon electrodes: a platform for electroanalytical and impedimetric biosensor applications<\/h4>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>This paper focuses on the development of a novel electrode based on boron-doped diamond nanosheet full-volume-enriched screen-printed carbon electrodes (BDDPE) for use as an impedimetric biosensor. Impedimetric biosensors ofer high sensitivity and selectivity for virus detection, but their use as point-of-care devices is limited by the complexity of nanomaterials\u2019 architecture and the receptor immobilisation procedures. The study presents a two-step modifcation process involving the electroreduction of diazonium salt at the BDDPE and the immobilisation of antibodies using zero-length cross-linkers for a selective impedimetric biosensor of <em>Haemophilus infuenzae<\/em> (Hi). The incorporation of diamond nanosheets into BDDPE leads to enhanced charge transfer and electrochemical behaviour, demonstrating greatly improved electrochemically active surface area compared with unmodifed screen-printed electrodes (by 44% and 10% on average for [Ru(NH<sub>3<\/sub>)<sub>6<\/sub>] Cl<sub>2<\/sub> and K<sub>3<\/sub>[Fe(CN)<sub>6<\/sub>], respectively). The presented sensing system shows high specifcity towards protein D in Hi bacteria, as confrmed by negative controls against potential interference from other pathogens, with an estimated tolerance limit for interference under 12%. The Hi limit of detection by electrochemical impedance spectroscopy was 1 CFU\/mL (measured at\u22120.13 V vs BDDPE pseudo-reference), which was achieved in under 10 min, including 5 min sample incubation in the presence of the analyte.<\/p>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"687\" height=\"255\" src=\"https:\/\/upturn.site\/pl\/wp-content\/uploads\/2023\/11\/11.png\" alt=\"Graficzny abstrakt\" class=\"wp-image-617\" srcset=\"https:\/\/upturn.site\/pl\/wp-content\/uploads\/2023\/11\/11.png 687w, https:\/\/upturn.site\/pl\/wp-content\/uploads\/2023\/11\/11-300x111.png 300w\" sizes=\"(max-width: 687px) 100vw, 687px\" \/><\/figure>\n\n\n\n<p><a href=\"https:\/\/link.springer.com\/article\/10.1007\/s00604-023-05991-w\">Pe\u0142na tre\u015b\u0107<\/a><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n","protected":false},"excerpt":{"rendered":"<p>Boron-doped diamond nanosheet volume-enriched screen-printed carbon electrodes: a platform for electroanalytical and impedimetric biosensor applications.<\/p>\n","protected":false},"author":1,"featured_media":495,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[12],"tags":[],"_links":{"self":[{"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/posts\/616"}],"collection":[{"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/comments?post=616"}],"version-history":[{"count":1,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/posts\/616\/revisions"}],"predecessor-version":[{"id":618,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/posts\/616\/revisions\/618"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/media\/495"}],"wp:attachment":[{"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/media?parent=616"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/categories?post=616"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/tags?post=616"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}