{"id":619,"date":"2023-11-27T08:48:00","date_gmt":"2023-11-27T06:48:00","guid":{"rendered":"http:\/\/upturn.site\/pl\/?p=619"},"modified":"2023-11-29T14:02:18","modified_gmt":"2023-11-29T12:02:18","slug":"publikacja-w-sensors-and-actuators-b-chemical","status":"publish","type":"post","link":"https:\/\/upturn.site\/pl\/publikacja-w-sensors-and-actuators-b-chemical\/","title":{"rendered":"Publikacja w Sensors and Actuators B: Chemical"},"content":{"rendered":"\n<h4 class=\"wp-block-heading has-theme-palette-8-background-color has-background\">Point-of-care testing for C-reactive protein in a sequential microfluidic device<\/h4>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>Point-of-care testing (POCT) devices are currently available for the detection of various important diseases owing to their easy-to-use, portable, and user-friendly nature. However, the multistep regent manipulation still impedes the performance of the device for end-users. To address this issue, we present a novel sequential flow-through microfluidic device that incorporates dual flow behaviors (fast-flow\/delayed) into a single device for assessing the risk of cardiovascular disease (CVDs) using C-reactive protein (CRP) as the model analyte. The device features a fast-flow channel for automated washing of unbound CRP antigens and a delayed channel for flow of a redox reagent for electrochemical analysis. It requires only a single buffer loading, resulting in a total analysis time of 15 min. To overcome challenges related to storage stability and the affinity of antibodies in the manufacturing process, we integrated the P3-CRP peptide as a new bioreceptor for CRP detection. The performance of both the P3-CRP peptide and antibodies was assessed and examined using chronocoulometry. The proposed device exhibited a limit of detection at 47 pg mL<sup>\u22121<\/sup> with a wide linear range (5 orders of magnitude). To validate its applicability, the device was tested with serum, plasma, and whole blood samples, yielding satisfactory results.<\/p>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"579\" src=\"https:\/\/upturn.site\/pl\/wp-content\/uploads\/2023\/11\/12-1024x579.jpg\" alt=\"\" class=\"wp-image-620\" srcset=\"https:\/\/upturn.site\/pl\/wp-content\/uploads\/2023\/11\/12-1024x579.jpg 1024w, https:\/\/upturn.site\/pl\/wp-content\/uploads\/2023\/11\/12-300x170.jpg 300w, https:\/\/upturn.site\/pl\/wp-content\/uploads\/2023\/11\/12-768x434.jpg 768w, https:\/\/upturn.site\/pl\/wp-content\/uploads\/2023\/11\/12-1536x868.jpg 1536w, https:\/\/upturn.site\/pl\/wp-content\/uploads\/2023\/11\/12.jpg 1567w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p><a href=\"http:\/\/doi.org\/10.1016\/j.snb.2023.134659\">Pe\u0142na tre\u015b\u0107<\/a><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n","protected":false},"excerpt":{"rendered":"<p>Point-of-care testing for C-reactive protein in a sequential microfluidic device.<\/p>\n","protected":false},"author":1,"featured_media":495,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[12],"tags":[],"_links":{"self":[{"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/posts\/619"}],"collection":[{"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/comments?post=619"}],"version-history":[{"count":1,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/posts\/619\/revisions"}],"predecessor-version":[{"id":621,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/posts\/619\/revisions\/621"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/media\/495"}],"wp:attachment":[{"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/media?parent=619"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/categories?post=619"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/upturn.site\/pl\/wp-json\/wp\/v2\/tags?post=619"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}